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Identify the wrong statement with regard to Restriction Enzymes.
The question specifically tests properties "with regard to RESTRICTION ENZYMES". Statements (1), (2), and (3) describe genuine properties of restriction enzymes (inspecting DNA length, cutting at palindromic sites, utility in genetic engineering). Statement (4) describes the action of a completely different enzyme — DNA Ligase! Official NTA Key: Option (4).
Restriction endonucleases are bacterial defense enzymes ("molecular scissors") utilized in recombinant DNA technology: - (1) Inspection: Each restriction enzyme scans the length of a DNA molecule to locate its specific recognition sequence. - (2) Palindromic Cleavage: Once bound, it cleaves both sugar-phosphate backbones within or near a specific palindromic nucleotide sequence. - (3) Genetic Engineering Utility: Because restriction digestion yields fragments with sticky single-stranded overhangs, it is an indispensable tool in genetic engineering to insert foreign genes into cloning vectors.
- Statement (4) states: "Sticky ends can be joined by using DNA ligases." While this is a true biological statement regarding DNA ligases, it does not describe an attribute or property of restriction enzymes; rather, it describes a downstream enzymatic step performed by an entirely separate class of enzymes (ligases). - Furthermore, restriction enzymes *produce* sticky ends by hydrolysis, they do not participate in ligase-mediated joining. - According to the Official NTA Final Answer Key (Booklet E1), Option (4) was officially designated as the intended correct answer.
Diagnose every choice: Know exactly why the right option works and why distractors fail.
This is a verbatim NCERT statement describing the scanning action of restriction endonucleases.
This is a true statement describing the catalytic hallmark of type II restriction endonucleases.
This is a true statement; restriction enzymes are fundamental tools in recombinant DNA construction.
Correct as the selected answer under official NTA key. Joining sticky ends is the property of DNA ligase, not restriction enzymes.
Candidates see that statement 4 is a true sentence and get confused. The question specifically asks for the wrong statement *with regard to Restriction Enzymes*. Statement 4 does not describe restriction enzymes.
"Restriction enzymes cut DNA at palindromes; joining sticky ends is the specific function of DNA ligase."
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